Related Research Publications by Cellastra’s Team:
Unger C, Skottman H, Blomberg P, Dilber MS, Hovatta O. Good manufacturing practice and clinical-grade human embryonic stem cell lines. Hum Mol Genet. 2008 Apr 15;17(R1):R48-53.
http://hmg.oxfordjournals.org/cgi/content/abstract/17/R1/R48
Skottman H, Narkilahti S, Hovatta O. Challenges and approaches to the culture of pluripotent human embryonic stem cells. Regen Med. 2007 May; 2(3):265-73.
Characterization of human embryonic stem cell lines by the International Stem Cell Initiative.
Nat Biotechnol. 2007 Jul;25(7):803-16. Epub 2007 Jun 17. (Outi Hovatta, an author for the consortium).
http://www.nature.com/nbt/journal/v25/n7/abs/nbt1318.html;jsessionid=3516370442A1CC04E20BF5139B8D8718
Unger C, Felldin U, Nordenskjöld A, Dilber MS, Hovatta O. Derivation of human skin fibroblast lines for feeder cells of human embryonic stem cells. Curr Protoc Stem Cell Biol. 2008 Jun;Chapter 1:Unit 1C.7.
http://www.ncbi.nlm.nih.gov/pubmed/18770630?ordinalpos=7&itool=EntrezSystem2.PEntrez.Pubmed.Pubmed_ResultsPanel.Pubmed_DefaultReportPanel.Pubmed_RVDocSum
Martin-Ibañez R, Unger C, Strömberg A, Baker D, Canals JM, Hovatta O. Novel cryopreservation method for dissociated human embryonic stem cells in the presence of a ROCK inhibitor. Hum Reprod. 2008 Dec;23(12):2744-54. http://www.ncbi.nlm.nih.gov/pubmed/18716037?ordinalpos=8&itool=EntrezSystem2.PEntrez.Pubmed.Pubmed_ResultsPanel.Pubmed_DefaultReportPanel.Pubmed_RVDocSum
Feki A, Bosman A, Dubuisson JB, Irion O, Dahoun S, Pelte MF, Hovatta O, Jaconi ME. Derivation of the first Swiss human embryonic stem cell line from a single blastomere of an arrested four-cell-stage embryo. Swiss Med Wkly. 2008 Sep 20;138(37-38):540-50.
http://www.smw.ch/docs/pdf200x/2008/37/smw-12385.pdf
Unger C, Kärner E, Treschow A, Stellan B, Felldin U, Concha H, Wendel M, Hovatta O, Aints A, Ahrlund-Richter L, Dilber MS. Lentiviral-Mediated HoxB4 Expression in Human Embryonic Stem Cells Initiates Early Hematopoiesis in a Dose-Dependent Manner but Does Not Promote Myeloid Differentiation.
Stem Cells. 2008 Jul 10.
http://stemcells.alphamedpress.org/cgi/content/abstract/2007-0876v1
Lappalainen RS, Narkilahti S, Huhtala T, Liimatainen T, Suuronen T, Närvänen A, Suuronen R, Hovatta O, Jolkkonen J. The SPECT imaging shows the accumulation of neural progenitor cells into internal organs after systemic administration in middle cerebral artery occlusion rats. Neurosci Lett. 2008 Aug 8;440(3):246-50.
Narkilahti S, Rajala K, Pihlajamaki H, Suuronen R, Hovatta O, Skottman H. Monitoring and analysis of dynamic growth of human embryonic stem cells: comparison of automated instrumentation and conventional culturing methods. Biomed Eng Online. 2007 Apr 12;6:11.
http://www.biomedical-engineering-online.com/content/6/1/11
van Harmelen V, Astrom G, Stromberg A, Sjolin E, Dicker A, Hovatta O, Ryden M. Differential lipolytic regulation in human embryonic stem cell-derived adipocytes. Obesity (Silver Spring). 2007 Apr;15(4):846-52.
Rajala K, Hakala H, Panula S, Aivio S, Pihlajamaki H, Suuronen R, Hovatta O, Skottman H. Testing of nine different xeno-free culture media for human embryonic stem cell cultures. Hum Reprod. 2007 Jan 24.
Assou S, Lecarrour T, Tondeur S, Strom S, Gabelle A, Marty S, Nadal L, Pantesco V, Reme T, Hugnot JP, Gasca S, Hovatta O, Hamamah S, Klein B, De Vos J. A meta-analysis of human embryonic stem cells transcriptome integrated into a web-based expression atlas. Stem Cells. 2007 Jan 4.
http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pubmed&pubmedid=17204602
Skottman H, Dilber MS, Outi Hovatta. The derivation of clinical-grade human embryonic stem cell lines. FEBS Lett. 2006 May 22;580(12):2875-8. Epub 2006 Apr 7. http://www.febsletters.org/article/PIIS0014579306004108/abstract
Skottman H, Stromberg AM, Matilainen E, Inzunza J, Outi Hovatta, Lahesmaa R. Unique gene expression signature by human embryonic stem cells cultured under serum-free conditions correlates with their enhanced and prolonged growth in an undifferentiated stage. Stem Cells. 2006 Jan;24(1):151-67. Epub 2005 Aug 11. http://stemcells.alphamedpress.org/cgi/content/full/24/1/151
Skottman H, Mikkola M, Lundin K, Olsson C, Stromberg AM, Tuuri T, Otonkoski T, Outi Hovatta, Lahesmaa R. Gene expression signatures of seven individual human embryonic stem cell lines. Stem Cells. 2005 Oct;23(9):1343-56. 2005 Aug 4. http://stemcells.alphamedpress.org/cgi/content/full/23/9/1343
Outi Hovatta, Meri S. Stem cell transplantation--a new era in medicine? Ann Med. 2005; 37(7):466-8.
http://www.informaworld.com/smpp/content?content=10.1080/07853890500404345
Inzunza J, Morani A, Cheng G, Warner M, Hreinsson J, Gustafsson JA, Hovatta O. Ovarian wedge resection restores fertility in estrogen receptor beta knockout (ERbeta-/-) mice. Proc Natl Acad Sci U S A. 2007 Jan 9;104(2):600-5.
Grinnemo KH, Kumagai-Braesch M, Mansson-Broberg A, Skottman H, Hao X, Siddiqui A, Andersson A, Stromberg AM, Lahesmaa R, Hovatta O, Sylven C, Corbascio M, Dellgren G. Human embryonic stem cells are immunogenic in allogeneic and xenogeneic settings. Reprod Biomed Online. 2006 Nov;13(5):712-24.
Nat R, Nilbratt M, Narkilahti S, Winblad B, Hovatta O, Nordberg A. Neurogenic neuroepithelial and radial glial cells generated from six human embryonic stem cell lines in serum-free suspension and adherent cultures. Glia. 2007 Mar;55(4):385-99.
Hovatta O. Derivation of human embryonic stem cell lines, towards clinical quality. Reprod Fertil Dev. 2006;18(8):823-8. Review.
http://www.publish.csiro.au/index.cfm?paper=RD06075
Zhang P, Kerkela E, Skottman H, Levkov L, Kivinen K, Lahesmaa R, Hovatta O, Kere J. Distinct sets of developmentally regulated genes that are expressed by human oocytes and human embryonic stem cells. Fertil Steril. 2007 Mar;87(3):677-90. Epub 2006 Nov 27.
Skottman H, Hovatta O. Culture conditions for human embryonic stem cells. Reproduction. 2006 Nov; 132(5):691-8. http://www.reproduction-online.org/cgi/content/full/132/5/691
Outi Hovatta, Skottman H. Feeder-free derivation of human embryonic stem-cell lines. Lancet. 2005 May 7-13;365(9471):1601-3.
Inzunza J, Gertow K, Stromberg MA, Matilainen E, Blennow E, Skottman H, Wolbank S, Ahrlund-Richter L, Outi Hovatta. Derivation of human embryonic stem cell lines in serum replacement medium using postnatal human fibroblasts as feeder cells. Stem Cells. 2005 Apr; 23(4):544-9.
Nat R, Outi Hovatta. In vitro neural differentiation of human embryonic stem cells. J Cell Mol Med. 2004 Oct-Dec;8(4):570-1. http://www.blackwell-synergy.com/doi/abs/10.1111/j.1582-4934.2004.tb00482.x
Koivisto H, Hyvarinen M, Stromberg AM, Inzunza J, Matilainen E, Mikkola M, Outi Hovatta, Teerijoki H. Cultures of human embryonic stem cells: serum replacement medium or serum-containing media and the effect of basic fibroblast growth factor. Reprod Biomed Online. 2004 Sep; (3):330-7.
Scott JE, Carlsson IB, Bavister BD, Outi Hovatta. Human ovarian tissue cultures: extracellular matrix composition, coating density and tissue dimensions. Reprod Biomed Online. 2004 Sep;9(3):287-93.
Imreh MP, Wolbank S, Unger C, Gertow K, Aints A, Szeles A, Imreh S, Outi Hovatta, Fried G, Dilber S, Ahrlund-Richter L. Culture and expansion of the human embryonic stem cell line HS181, evaluated in a double-color system. Stem Cells Dev. 2004 Aug;13(4):337-43.
IB, Laitinen MP, Scott JE, Louhio H, Velentzis L, Tuuri T, Aaltonen J, Ritvos O, Winston RM, Outi Hovatta. Kit ligand and c-Kit are expressed during early human ovarian follicular development and their interaction is required for the survival of follicles in long-term culture. Reproduction. 2006 Apr; 131(4):641-649.
Mirza M, Hreinsson J, Strand ML, Outi Hovatta, Soder O, Philipson L, Pettersson RF, Sollerbrant K. Coxsackievirus and adenovirus receptor (CAR) is expressed in male germ cells and forms a complex with the differentiation factor JAM-C in mouse testis.Exp Cell Res. 2006 Apr 1;312(6):817-30.
Outi Hovatta. Methods for cryopreservation of human ovarian tissue. Reprod Biomed Online. 2005 Jun;10(6):729-34. Review.
Zhang P, Louhio H, Tuuri T, Sjoberg J, Hreinsson J, Telfer EE, Outi Hovatta. In vitro effect of cyclic adenosine 3', 5'-monophosphate (cAMP) on early human ovarian follicles. J Assist Reprod Genet. 2004 Aug;21(8):301-6.
Hreinsson J, Rosenlund B, Fridstrom M, Ek I, Levkov L, Sjoblom P, Outi Hovatta. Embryo transfer is equally effective at cleavage stage and blastocyst stage: a randomized prospective study. Eur J Obstet Gynecol Reprod Biol. 2004 Dec 1;117(2):194-200.
Inzunza J, Sahlen S, Holmberg K, Stromberg AM, Teerijoki H, Blennow E, Outi Hovatta, Malmgren H. Comparative genomic hybridization and karyotyping of human embryonic stem cells reveals the occurrence of an isodicentric X chromosome after long-term cultivation. Mol Hum Reprod. 2004 Jun;10(6):461-6. Epub 2004 Mar 25.
Outi Hovatta. Cryopreservation and culture of human ovarian cortical tissue containing early follicles. Eur J Obstet Gynecol Reprod Biol. 2004 Apr 5;113 Suppl 1:S50-4. Review.
Lindeberg M, Outi Hovatta, Ahrlund-Richter L. Real-time reverse transcription-polymerase chain reaction analysis of translation initiation factor 1A (eIF-1A) in human and mouse preimplantation embryos. Reprod Biomed Online. 2004 Mar;8(3):338-43.
Scott JE, Zhang P, Outi Hovatta. Benefits of 8-bromo-guanosine 3',5'-cyclic monophosphate (8-br-cGMP) in human ovarian cortical tissue culture. Reprod Biomed Online. 2004 Mar;8(3):319-24.
Hreinsson J, Zhang P, Swahn ML, Hultenby K, Outi Hovatta. Cryopreservation of follicles in human ovarian cortical tissue. Comparison of serum and human serum albumin in the cryoprotectant solutions. Hum Reprod. 2003 Nov;18(11):2420-8.
Ringden O, Le Blanc K, Outi Hovatta. Transplantation of embryonic stem cells: possibilities and challenges. Transplantation. 2003 Oct 15;76(7):1011-2.
Stavreus-Evers A, Outi Hovatta, Eriksson H, Landgren BM. Development and characterization of an endometrial tissue culture system. Reprod Biomed Online. 2003 Sep;7(2):243-9.
Hreinsson J, Rosenlund B, Friden B, Levkov L, Ek I, Suikkari AM, Outi Hovatta, Fridstrom M. Recombinant LH is equally effective as recombinant hCG in promoting oocyte maturation in a clinical in-vitro maturation programme: a randomized study.
Hum Reprod. 2003 Oct;18(10):2131-6.
Outi Hovatta, Mikkola M, Gertow K, Stromberg AM, Inzunza J, Hreinsson J, Rozell B, Blennow E, Andang M, Ahrlund-Richter L. A culture system using human foreskin fibroblasts as feeder cells allows production of human embryonic stem cells.
Hum Reprod. 2003 Jul;18(7):1404-9.
Bjuresten K, Outi Hovatta. Donation of embryos for stem cell research--how many couples consent? Hum Reprod. 2003 Jun;18(6):1353-5.
Outi Hovatta. Cryobiology of ovarian and testicular tissue. Best Pract Res Clin Obstet Gynaecol. 2003 Apr;17(2):331-42. Review.
Outi Hovatta. A North European perspective in assisted reproduction-new legislation is coming in Finland and in Sweden. Reprod Biomed Online. 2002 Mar-Apr;4(2):197-8.
Malmgren H, Sahlen S, Inzunza J, Aho M, Rosenlund B, Fridstrom M, Outi Hovatta, Ahrlund-Richter L, Nordenskjold M, Blennow E. Single cell CGH analysis reveals a high degree of mosaicism in human embryos from patients with balanced structural chromosome aberrations. Mol Hum Reprod. 2002 May;8(5):502-10.
Hreinsson JG, Scott JE, Rasmussen C, Swahn ML, Hsueh AJ, Outi Hovatta. Growth differentiation factor-9 promotes the growth, development, and survival of human ovarian follicles in organ culture. J Clin Endocrinol Metab. 2002 Jan;87(1):316-21.
Outi Hovatta, Ahrlund-Richter L. [Ethical aspects of stem cell research. Legislation and guidelines in Europe] Lakartidningen. 2001 Aug 22;98(34):3515-9.
Fridstrom M, Ahrlund-Richter L, Iwarsson E, Malmgren H, Inzunza J, Rosenlund B, Sjoblom P, Nordenskjold M, Blennow E, Outi Hovatta. Clinical outcome of treatment cycles using preimplantation genetic diagnosis for structural chromosomal abnormalities. Prenat Diagn. 2001 Sep;21(9):781-7.
Outi Hovatta. Cryopreservation of testicular tissue in young cancer patients.Hum Reprod Update. 2001 Jul-Aug;7(4):378-83. Review.
Van den Broecke R, Liu J, Handyside A, Van der Elst JC, Krausz T, Dhont M, Winston RM, Outi Hovatta. Follicular growth in fresh and cryopreserved human ovarian cortical grafts transplanted to immunodeficient mice. Eur J Obstet Gynecol Reprod Biol. 2001 Aug;97(2):193-201.
Outi Hovatta. Cryopreservation and culture of human primordial and primary ovarian follicles. Mol Cell Endocrinol. 2000, Nov;169(1-2):95-7. Review.
Outi Hovatta. Cryopreservation of testicular tissue. Mol Cell Endocrinol. 2000 Nov 27;169(1-2):113-5. Review.
Neerja Sethi, Palefsky J. Transcriptional profiling of dysplastic lesions in K14-HPV16 transgenic mice using laser microdissection. FASEB J. 2004 Aug;18(11):1243-5. Epub 2004 Jun 4.
http://www.fasebj.org/cgi/content/full/18/11/1243
Neerja Sethi, Palefsky J. Treatment of human papillomavirus (HPV) type 16-infected cells using herpes simplex virus type 1 thymidine kinase-mediated gene therapy transcriptionally regulated by the HPV E2 protein. Hum Gene Ther. 2003 Jan 1;14(1):45-57.
http://www.liebertonline.com/doi/abs/10.1089/10430340360464705
Neerja Sethi, M. Cristina Monteagudo, Douglas Koshland, Eileen Koshland, and Daniel J. Burke. 1991. The cdc20 gene product of Saccharomyces cerevisiae, a beta-transducin homologue, is required for a subset of microtubule-dependent processes. Molecular and Cell Biology. Vol. 11, No. 11:5592-5602.
http://www.pubmedcentral.gov/articlerender.fcgi?artid=361930
D. J. Burke and Neerja Sethi. 1997. A change in sister chromatid behavior precedes nuclear division in Saccharomyces cerevisiae. Molecular General Genetics. Vol. 254. No.1, 21-28.
Kent Persson, Hamby K, Ugozzoli LA. Four-color multiplex reverse transcription polymerase chain reaction--overcoming its limitations. Anal Biochem. 2005 Sep 1;344(1):33-42.
Teresa Rubio, William Heiser, Joseph Terefe, Kent Persson, Sean Baker, Keith Hamby and Julie Moore. Bio-Rad Laboratories Hercules, CA. Improved Delivery of siRNA using siLentFectTM Lipid Reagent. Beyond Genome 2005, June 13 - 16, 2005, San Francisco, CA.
Kent Persson, PhD. Multiplex Real-Time PCR. Gene Expression Assays on the iCycler iQ. ®. http://www.wzw.tum.de/gene-quantification/qpcr2004/pub/Srere.pdf
